| Active Transport Essay, Research Paper
Since the cell membrane is somewhat permeable to sodium ions, simple diffusion would result in a net movement of
sodium ions into the cell, until the concentrations on the two sides of the membrane became equal. Sodium actually
does diffuse into the cell rather freely, but as fast as it does so, the cell actively pumps it out again, against the
concentration difference.
The mechanism by which the cell pumps the sodium ions out is called active transport. Active transport requires the
expenditure of energy for the work done by the cell in moving molecules against a concentration gradient. Active
transport enables a cell to maintain a lower concentration of sodium inside the cell, and also enables a cell to
accumulate certain nutrient inside the cell at concentrations much higher than the extracellular concentrations.
The exact mechanism of active transport is not known. It has been proposed that a carrier molecule is involved,
which reacts chemically with the molecule that is to be actively transported. This forms a compound which is
soluble in the lipid portion of the membrane and the carrier compound then moves through the membrane against
the concentration gradient to the other side. The transported molecule is then released, and the carrier molecule
diffuses back to the other side of the membrane where it picks up another molecule. This process requires energy,
since work must done in transporting the molecule against a diffusion gradient. The energy is supplied in the form of
ATP.
The carrier molecules are thought to be integral proteins; proteins which span the plasma membrane. These proteins
are specific for the molecules they transport.
Chemiosmosis
Populating the inner membrane of the mitochondrion are many copies of a protein complex called an ATP synthase,
the enzyme that actually makes ATP! It works like an ion pump running in reverse. In the reverse of that process, an
ATP synthase uses the energy of an existing ion gradient to power ATP synthesis. The ion gradient that drives
oxidative phosphorylation is a proton (hydrogen ion) gradient; that is, the power source for the ATP syntheses is a
difference in the concentration of H+ on opposite sides of the inner mitochondrial membrane. We can also think of
this gradient as a difference in pH, since pH is a measure of H+ concentration.
The function of the electron transport chain is to generate and maintain an H+ gradient. The chain is an energy
converter that uses the exergonic flow of electrons to pump H+ across the membrane, from the matrix into the
intermembrane space. The H+ leak back across the membrane, diffusing down its gradient. But the ATP synthases
are the only patches of the membrane that are freely permeable to H+. The ions pass through a channel in an ATP
synthase, and the complex of proteins functions as a mill that harnesses the exergonic flow of H ‘ to drive the
phosphorylation of ATP Thus, an H+ gradient couples the redox reactions of the electron transport chain to ATP
synthesis. This coupling mechanism for oxidative phosphorylation is called chemiosmosis, a term that highlights the
relationship between chemical reactions and transport across the membrane. We have previously used the word
osmosis in discussing water transport, but here the word refers to the pushing of H+ across a membra!
ne.
Certain members of the electron transport chain must accept and release protons (H+) along with electrons,
while other carriers transport only electrons. Therefore, at certain steps along the chain, electron transfers cause H+
to be taken up and released back into he surrounding solution. The electron carriers are spatially arranged in the
membrane in such a way that H+ is accepted from the mitochondrial matrix and deposited – the intermembrane
space. The H+ gradient that results is referred to as a proton-motive force, emphasizing the capacity of the gradient
to perform work. The force drives H+ back across the membrane through the
specific H+ channels provided by ATP synthase complexes. How the ATP synthase uses the downhill H+ current to
attach inorganic phosphate to ADP is not yet known. The hydrogen ions may participate directly in the reaction, or
they may induce a conformation change of the ATP synthase that facilitates phosphorylation. Research has revealed
the general mechanism of energy coupling by chemiosmosis, but many details of the process are still uncertain. The
key feature of chemiosmosis is: It is an energy-coupling mechanism that uses exergonic redox reactions to store
energy in the form of an H+ gradient, which then drives other kinds of work, including ATP synthesis.
Chemiosmosis is not unique to mitochondria. Chloroplasts also use the mechanism to generate ATP during
photosynthesis; the main difference is that light drives electrons along an electron transport chain. Bacteria, which
lack both mitochondria and chloroplasts , generate H + gradients across their plasma membranes. They!
then tap the proton-motive force to make ATP to pump nutrients and waste products across the membrane, and even
to move by rotating their flagella.
Fermentation
How can food be oxidized without oxygen? Remember, oxidation refers to the loss of electrons to any electron
acceptor, not just to oxygen. Glycolysis oxidizes glucose to two molecules of pyruvate. The oxidizing agent of
glycolysis is NAD+, not oxygen. The oxidation of glucose is exergonic, and glycolysis uses some of the energy
made available to produce two ATPs (net) by substrate-level phosphorylation. If oxygen is present, then additional
ATP is made by oxidative phosphorylation when NADH passes electrons removed from glucose to the electron
transport chain. But glycolysis generates two ATPs whether oxygen is present or not?that is, whether conditions
are aerobic or anaerobic.
Anaerobic catabolism of organic nutrients can occur by fermentation. Fermentation can generate ATP by substrate
level phosphorylation, as long as there is a sufficient supply of NAD+ to accept electrons during the oxidation step
of glycolysis. Without some mechanism to recycle NAD+ from NADH, glycolysis would soon deplete the cell’s
pool of NAD+ and shut itself down for lack of an oxidizing agent. Under aerobic conditions, NAD+ is recycled
productively from NADH by the transfer of electrons to the electron transport chain. The anaerobic alternative is to
transfer electrons from NADH to pyruvate, the end product of glycolysis.
Fermentation consists of gIycolysis plus reactions that regenerate NAD+ by transferring electrons from NADH to
pyruvate or derivatives of pyruvate. There are many types of fermentation, differing in the waste products formed
from pyruvate. Two common types are alcohol fermentation and lactic acid fermentation.
In alcohol fermentation, pyruvate is converted to ethanol, or ethyl alcohol, in two steps. The first step releases
carbon dioxide from the pyruvate, which is converted to the two-carbon compound acetaldehyde. In the second step,
acetaldehyde is reduced by NADH to ethyl alcohol. This regenerates the supply of NAD+ needed for glycolysis.
Alcohol fermentation by yeast, a fungus, is used in brewing and wine making. Many bacteria also carry out alcohol
fermentation under anaerobic conditions. During lactic acid fermentation pyruvate is reduced directly by NADH to
form lactate as a waste product, with no release of CO2. (Lactate is the ionized form of lactic acid.) Lactic acid
fermentation by certain fungi and bacteria is used in the dairy industry to make cheese and yogurt. Acetone and
methyl alcohol are among the by-products of other types of microbial fermentation that are commercially important.
Human muscle cells make ATP by lactic acid fermentation when oxygen is scarce. This occurs during the early
stages of strenuous exercise, when sugar catabolism for ATP production outpaces the muscle’s supply of oxygen
from the blood. Under these conditions, the cells switch from aerobic respiration to fermentation. The lactate that
accumulates as a waste product may cause muscle fatigue and pain, but it is gradually carried away by the blood to
the liver. Lactate is converted back to pyruvate by the liver cells.
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